Cover Image

On the role of medium components in bio-surfactant production from Achromobacter xylos GSR21

Golamari Siva Reddy, Kamma Srinivasulu, Botlagunta Mahendran, Ronda Srinivasa Reddy

Abstract


This paper, for the first time, reports the optimization of the critical medium components for bio-surfactant production from achromobacter xylos strain GSR21 using statistical experimental design. Response surface methodology (RSM) was employed to determine the optimal levels of process variables (agar powder, yeast extract, FeSO47H2O, and KH2PO4). Central composite design (CCD) of RSM was used to study the four variables at five levels, and bio-surfactant concentration was measured as response. Regression coefficients were calculated by regression analysis, and the model equation was determined. R2 value for bio-surfactant (g/L) was tested to be 0.7222, indicating that the model fitted well with the experimental results. Verification of the mathematical model was conducted by performing the experiment with the predicted optimized values, and bio-surfactant yield was found to be 9.69 g/L. Validation of the predicted model was fitted 96.9% with the experimental results conducted under the optimum conditions. Agar powder and yeast extract was identified as efficient components for bio-surfactant (achromobacter xylos GSR21) production.


Full Text:

PDF

References


Abbasi H, Sharafi H, Alidost L, Bodagh A, Zahiri HS, Noghabi KA., Response surface optimization of biosurfactant produced by Pseudomonas aeruginosa MA01 isolated from spoiled apples. Prep. Biochem. Biotechnol., 43: 398-414, 2013.

Bas D, Boyaci IH., Modeling and optimization I: Usability of response surface methodology. J. Food Eng., 78: 836-845, 2007.

Besson F, Michel G., Biosynthesis of iturin and surfactin by Bacillus subtilis. Evidence for amino acid activating enzymes. Biotechnol. Lett., 14: 1013-1018,1992.

Casas JA, García-Ochoa F., Sophorolipid production by Candida bombicola: medium composition and culture methods. J. Biosci. Bioeng., 88: 488-494,1999.

Chakravarti R, Sahai V., Optimization of compactin production in chemically defined production medium by Penicillium citrinum using statistical methods. Process Biochem., 38: 481-486,2002.

Desai JD, Banat IM., Microbial production of biosurfactants and their commercial potential. Microbiol. Mol. Biol. Rev., 61: 41-64, 1997.

Gandhimathi R, Seghal Kiran G, Hema TA, Selvin J, Rajeetha Raviji T, Shanmughapriya S. , Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10. Bioprocess Biosyst. Eng., 32: 825-835, 2009.

Ghojavand H, Vahabzadeh F, Mehranian M, Radmehr M, Shahraki KA, Zolfagharian F, Emadi MA, Roayaei E., Isolation of thermotolerant, halotolerant, facultative biosurfactant producing bacteria. Appl. Microbiol. Biotechnol., 80: 1073-1085, 2008.

Guerra de Oliveira J, Garcia-Cruz CH., Properties of a biosurfactant produced by Bacillus pumilus using vinasse and waste frying oil as alternative carbon sources. Braz. Arch. Biol. Technol., 56: 155-160,2013.

Haaland PD. 1989. Experimental design in biotechnology. Dekker, New York.

Hickey AM, Gordon M, Dodson ADW, Kelly CT, Doyle EM. Effect of surfactants on fluoranthene degradation by Pseudomonas alcaligenes PA-10. Appl. Microbiol. Biotechnol., 74: 851-856,2007.

Khayati G, Gilani HG, Kazemi M., The effect of olive cake types on lipase production by isolated Rhizopus sp. and process statistical optimization. J. Biosci. Biotechnol., 2: 45-55, 2013.

Kim H, Yoon B, Lee C, Suh H, Katsuragi T, Tani Y., Production and properties of a lipopeptide biosurfactant from Bacillus subtilis C9. J. Ferm. Bioeng., 84: 41-46,1997.

Makkar RS, Cameotra SS. Effects of various nutritional supplements on biosurfactant production by a strain of Bacillus subtilis at 45°C. J. Surf. Deter., 5: 11-18, 2002.

Mohan PK, Mukhla G, Yanful EK., Biokinetics of biodegradation of surfactants under aerobic, anoxic and anaerobic conditions. Water Res., 40: 533-540, 2006.

Mulligan CN., Environmental application for biosurfactants. Environ. Poll., 133: 183-198,2005.

Myers RH, Montgomery DC. 1995. Response surface methodology, process and product optimization using design experiments. John Wiley & Sons, New York.

Neto DM, Meira JA, de Araujo JM, Mitchel DA, Krieger N., Optimization of the production of rhamnolipids by Pseudomonas aeruginosa UFPEDA 614 in solid-state culture. Appl. Microbiol. Biotechnol., 81: 441-448,2008.

Priya R, Suresh Kumar S, Gupta R., Concomitant production and downstream processing of alkaline protease and biosurfactant from Bacillus licheniformis RG1:Bioformulation as detergent additive. Process Biochem., 40: 3352-3359, 2005.

Sandrin C, Peypoux F, Michel G. Coproduction of surfactin and iturin A lipopeptides with surfactant and antifungal properties by Bacillus subtilis. Biotechnol. Appl. Biochem., 12: 370–375, 1990.

Sanket J, Chirag B, Anjana JD., Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B. Bioresour. Technol., 99: 4603-4608, 2008.

Sasidharan Satheesh Kumar , Govindasamy Sharmila, Chandrasekaran Muthukumaran, Krishnamurthi Tamilarasan and Margavelu Gopinath., Statistical optimization of critical medium components for biosurfactant production by Bacillus subtilis., J. BioSci. Biotechnol. 4(2): 123-129, 2015.

Singh A, Van Hamme JD, Ward OP., Surfactants in microbiology and biotechnology: Part 2. Application aspects. Biotechnol. Adv., 25: 99–121, 2007.

Tamilarasan K, Dharmendira Kumar M. Optimization of medium components and operating conditions for the production of solvent-tolerant lipase by Bacillus sphaericus MTCC 7542. Afr. J. Biotechnol., 10: 15051-15057, 2011.




DOI: http://dx.doi.org/10.21967/jbb.v3i4.172

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Golamari Siva Reddy, Kamma Srinivasulu, Botlagunta Mahendran, Ronda Srinivasa Reddy

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.