Cover Image

Determination of Furfural and Hydroxymethyl furfural by UV Spectroscopy in ethanol-water hydrolysate of Reed

Haiyang Zhang, Qingwei Ping, Jian Zhang, Na Li


In this paper a quick method was developed to determine separate furfural and HMF concentrations simultaneously in ethanol-water hydrolysate of reed based on UV spectroscopy. Acid soluble lignin and other interfering substances were first removed by distillation as residue. The distillate was then used for the determination of furfural and HMF by measuring the maximum absorption wavelength and the absorbance at the wavelength. Results showed that the maximum absorption wavelength of the characteristic peak correlated well with the composition of furfural and HMF mixture in an ethanol-water solution, and the absorbance at the maximum absorption wavelength also had an excellent linear relationship with the sum concentration of furfural and HMF in the solution. The separate concentrations of furfural and HMF in a mixture solution could be determined by applying these correlations.

Full Text:



Nirmal Uppugundla1, Leonardo da Costa Sousa1, Shishir PS Chundawat, et al. A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover [J]. Biotechnology for Biofuels 2014, 7: 72.

Xuejun Pan, Claudio Arato, Neil Gilkes, etal. Biorefining of Softwoods Using Ethanol Organosolv Pulping: Preliminary Evaluation of Process Streams for Manufacture of Fuel-Grade Ethanol and Co-Products: Biotechnology and Bioengineering 2005;90: 473–481

Paraskevi Panagiotopoulou, Dionisios G. Vlachos. Liquid phase catalytic transfer hydrogenation of furfural over a Ru/C catalyst.Applied Catalysis A: General 2014; 480: 17–24

Yu Su, Heather M. Brown, Xiwen Huang, Xiao-dong Zhou. Single- step conversion of cellulose to 5-hydroxymethylfurfural (HMF), a versatile platform chemical.

Hua Jin, Yanshuo Li, Xinlei Liu. Recovery of HMF from aqueous solution by zeolitic imidazolate frameworks. Chemical Engineering Science 2015; 124: 170-178

Cristina Sánchez, Luis Serrano, Ma Angeles Andres. Furfural production from corn cobs autohydrolysis liquors by microwave technology. Industrial Crops and Products; 42:513-519

Wei Liu, John Holladay. Catalytic conversion of sugar into hydroxymethylfurfural in ionic liquids. Catalysis Today 2013; 200:106-116

LU Qiang, LIAO Hang-tao, ZHANG Yang. Reaction mechanism of low-temperature fast pyrolysis of fructose to produce 5-hydroxymethyl furfural. Journal of fuel chemistry and technology 2013; 41:1070-1076

F.López, M.T.García, M.J.Feria. Optimization of furfural production by acid hydrolysis of Eucalyptus globulus in two stages. Chemical Engineering Journal 2014; 240:195-201

Xinli Tong, Yanhua Wang, Guangxia Nie. Selective Dehydration of Fructose and Sucrose to 5-Hydroxymethyl-2-Furfural with Heterogeneous Ge (IV) Catalysts. Environmental Progress & Sustainable Energy 2015; 34:207-210.

S. Hyvärinen, J.P. Mikkola, D. Yu. Murzin. Sugars and sugar derivatives in ionic liquid media obtained from lignocellulosic biomass: Comparison of capillary electrophoresis and chromatographic analysis. Catalysis Today 2014; 223:18-24.

Merike Vaher, Kati Helmja, Andres Käsper. Capillary electrophoretic monitoring of hydrothermal pre-treatment and enzymatic hydrolysis of willow: Comparison with HPLC and NMR. Catalysis Today 2012; 196:34-41

Jinzhang Gao, Hongxia Dai, Wu Yang. Determination of furfural in an oscillating chemical reaction using an analyte pulse perturbation technique. Ana Bioanal Chem 2006; 384:1438-1443

Qiang Lu, Chang-qing Dong, Xu-ming Zhang. Selective fast pyrolysis of biomass impregnated with ZnCl2 to produce furfural: Analytical Py-GC/MS study. Journal of Analytical and Applied Pyrolysis 2011; 90:204-212

Ján Hrivňák, Peter Tölgyessy, Soňa Figedyová, Svetozár Katuščák. A Solid-Phase Microcolumn Extraction Method for the Analysis of Acetic Acid and Furfural Emitted from Paper. Chromatographia 2009; 70: 619–622

Xun Hu, Richard Gunawan, Daniel Mourant. Acid-catalysed reactions between methanol and the bio-oil from the fast pyrolysis of mallee bark. Fuel 2012; 97:512-522

C. Petisca, A.R. Henriques, T. Pe´rez-Palacios. Assessment of hydroxymethylfurfural and furfural in commercial bakery products. Journal of Food Composition and Analysis 2014; 33:20-25

Debora Nabarlatz, Anna Ebringerová, Daniel Montané. Autohydrolysis of agricultural by-products for the production of xylo-oligosaccharides. Carbohydrate Polymers 2007; 69:20-28

Gil garrote, Herminia Domínguez, Juan Carlos Parajó. Generation of xylose solutions from Eucalyptus globulus wood by autohydrolysis-posthydrolysis processes: posthydrolysis kinetics. Bioresource Technology 2001; 79:155-164

Jill Jensen, Juan Mori nelly, Anw ar Aglan. Kinetic Characterization of Biomass Dilute Sulfuric Acid Hydrolysis:Mixtures of Hardwoods, Softwood, and Switchgrass. Environmental and energy engineering 2008; 54:1637-1645

Debora Nabarlatz, Xavier Farriol, Daniel Montane. Kinetic Modeling of the Autohydrolysis of Lignocellulosic Biomass for the Production of Hemicellulose-Derived Oligosaccharides. Industrial engineering chemistry research 2004; 43: 4124-4131

Eliana V. Canettieri, George J. M. Rocha, George J. M. Rocha. Evaluation of the Kinetics of Xylose Formation from Dilute Sulfuric Acid Hydrolysis of Forest Residues of Eucalyptus grandis. Industrial engineering chemistry research 2007; 46: 1938-1944

Jenny Sabrina Gütsch, Tiia Nousiainen, Herbert Sixta. Comparative evaluation of autohydrolysis and acid-catalyzed hydrolysis of Eucalyptus globulus wood. Bioresource Technology 2012; 109: 77–85

M. Sefik Tunc, Juben Chheda, Evert van der Heide. Two-Stage Fractionation and Fiber Production of lignocellulosic Biomass for Liquid Fuels and Chemicals. Industrial engineering chemistry research 2013; 52: , 13209 −13216

Debora Nabarlatz, Xavier Farriol, Daniel Montane. Autohydrolysis of Almond Shells for the Production of Xylo-oligosaccharides: Product Characteristics and Reaction. Industrial engineering chemistry research 2005; 44:7746-7755.

Alfredo Martinez, Maria E. Rodriguez, Sean W. York, et al. Use of UV Absorbance To Monitor Furans in Dilute AcidHydrolysates of Biomass [J]. Biotechnology Progress, 2000, 16:637-641。

C.W. Dence. Determination of lignin [J]. Methods in Lignin Chemistry, 1992; 33–61.



  • There are currently no refbacks.

Copyright (c) 2017 Journal of Bioresources and Bioproducts